‍ModaFlow™, accelerates the ongoing therapeutic Antibody Revolution!

Since muromonab-CD3 (Orthoclone OKT3) was approved by the FDA in 1986, monoclonal antibodies became increasingly important amongst the therapeutics arsenal in the 2000s, particularly in immunotherapy for diseases like cancer, autoimmune disorders, and infections.

Since then, blockbusters spread with adalimumab developed against rheumatoid arthritis, checkpoint inhibitors like pembrolizumab against PD-L1 positive cancers, and bamlanivimab that was rapidly developed and authorised for emergency use to treat COVID-19.

In 2024, they are around 30 monoclonal antibody therapeutics in Phase III clinical trials or have been reviewed by regulatory agencies, demonstrating that therapeutic antibodies have moved from experimental tools into the cornerstone of modern medicine.

In the early phases of the discovery process, the challenge is to screen entire immune repertoires with fast, convenient, and efficient methods.

Traditional and alternative methods have limitations in terms of efficiency and feasibility.

High-throughput single-cell technology, especially droplet microfluidics, has revolutionised the screening process, allowing for the rapid and comprehensive analysis of antibody-producing cells.

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Modern High-Throughput Techniques

Droplet microfluidics allows the encapsulation of single B cells with reagents into tiny droplets of few tens of picoliters, offering a high-throughput platform for antibody screening. These miniaturised assays are designed to facilitate the discovery of antibodies (IgG & VHH) against both soluble and insoluble target antigens, addressing the complexities associated with challenging targets.

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Droplet  Microfluidics

• Enables  high-throughput screening of millions of antibody-producing cells (B-cells, plasma cells, or plasmablasts).

• Screening is based on the actual secretion of antibodies, improving efficiency and throughput.

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The ModaFlow^TM instrument

ModaFlow^TM is a revolutionary robust, reproducible, all-in-one integrated instrument designed to perform high-throughput screening of antibody producing cells while offering the maximal versatility required for productive R&D discovery work.

The platform is a unique combination of robust and optimised hardware & software modules (high-end microfluidics, optics, software, user interface, …) making its regulatory registration easier.

Per se, ModaFlow™ brings a comprehensive, high-end microfluidic and analytical laboratory within reach for antibody therapeutic researchers, all within a single bench-top instrument. It is compatible with single-use cartridges, off-the-shelf from LiveDrop’s catalog or custom-made.

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Specific advantages of using ModaFlow^TM for Cell-Line development

• LiveDrop’s generic label-free bioassay technology adaptable to any immune target (*).
• Comprehensive Immune Repertoire Analysis: ModaFlow^TM permits the development of high-throughput workflows enabling the analysis of entire immune repertoires.
• Broad range of targets both soluble and insoluble can be detected, enhancing its applicability to a wide range of applications.
• Single-cell analysis allows characterising the phenotype and linking it to the genotype thanks to OMICS.
• Versatile multi-parametric functional assays: ModaFlow^TM supports various assays, e.g., ADCC, CDC, ADCP, binder agonist / antagonist to ensure the discovery     of antibodies with desired therapeutic functions.
• Fast: several runs of screening, incubation, sorting and single-cell seeding can be     managed in one day.
• Simple and flexible: a single instrument with one chip for single-cell encapsulation (step 1) and one chip for screening and sorting (step 2).
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  (*) An exclusive, proprietary technology.

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Tailored cell development support: LiveDrop provides systematic expert support, ensuring users are accompanied when setting up new biological assays:

• Personalised assistance for setup and optimisation.
• Comprehensive training programs.
• Collaborative community through webinars and forums.

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Generic advantages of using ModaFlow^TM

Key Features of the ModaFlow^TM
The Modaflow^TM instrument revolutionises the microfluidic biology landscape by offering a fully integrated, compact, and versatile solution that rivals the capabilities of traditional, space-consuming setups. An entire microfluidic biology lab has been compacted into a portable unit making it a highly valuable tool for researchers and innovation enthusiasts, driving efficiency.

For droplet biology beginners or advanced experts, the Modaflow^TM instrument is the dream solution in all domains, easy to set up and to operate.

• Compact Integration:
  • Benchtop: all necessary components for microfluidic experiments are condensed into a compact unit, freeing up laboratory space and reducing the clutter associated with typical microfluidic setups.
  • Ease of use: the integration eliminates the need for complex tubing and multiple external control panels, simplifying the operation and maintenance.
• Versatility: within one single instrument, ModaFlow^TM performs:
• Encapsulation of individual cells or pairs of cell population, with medium and or reagents
• Droplet reinjection using different kind of cartridges
• Fluorescence screening and sorting
• Real-time  imaging: Includes a camera for real-time observation and control of processes within microfluidic chips, providing immediate feedback for precise adjustments. Synchronising imaging with sorting enhances quality control.
• Robust design:  engineered for endurance, the Modaflow^TM can withstand the demands of high-throughput and long-duration experiments without compromising performance.
• Efficiency: ensures consistent operation, no dead volume and no sample loss minimising downtime and enhancing productivity in research and development environments.
  

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Don't wait accelerating your ongoing functional antibody R&D with ModaFlow™!

We invite you to leverage ModaFlow's advanced microfluidic technology to streamline your antibody discovery and development processes. We will help you achieve breakthroughs in therapeutic antibody development with unparalleled speed and precision.

Contact us immediately at info@livedrop-bio.com or +32 378 63 31 to initiate a live interaction.

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